AFM-in-SEM

for In-Situ Correlative Microscopy

LiteScope

We introduce the game-changing Atomic Force

Microscope (AFM) for seamless integration into

Scanning Electron Microscope (SEM), which

unlocks new possibilities for in-situ correlative

microscopy

Why AFM-in-SEM?

Scanning electron microscopy and atomic

force microscopy are the two most used and

complementary techniques for sample analysis

in the sub-nanometer range. The integration of

AFM into SEM merges the strengths of both


techniques, resulting in extremely time-efficient

workflow and enabling complex sample analysis

that was difficult or practically impossible

by conventional, separate AFM and SEM

instrumentation.

Key technology benefits

Correlative multimodal

sample analysis

Cutting-edge CPEM technology

allows the simultaneous

acquisition of AFM and SEM

data and their seamless

correlation

In-situ sample

characterization

In-situ conditions inside the SEM

ensure sample analysis at the

same time, in the same place

and under the same conditions.

Precise localization

of the region of interest

Extremely precise and time

saving approach uses SEM to

navigate the AFM tip to the

region of interest, enabling its

fast & easy localization.

We merge the forces of AFM and SEM

AFM

  • Precise 3D topography
  • High resolution (to sub-nm)
  • Mechanical properties
  • Electrical properties
  • Magnetic properties

SEM

  • Fast 2D imaging
  • Viewfield range (mmnm)
  • Surface modification
  • Elemental composition
  • Crystal structures

How does it work?

On the sample, the electron beam points close

to the AFM tip with a constant offset. They both

remain static, while the sample is scanned with the

LiteScopes piezo scanner.

This way, data from both microscopes can be

acquired at the same time, in the same place,

and under the same conditions.

Application areas

LiteScope offers users unprecedented

possibilities in sample analysis and advanced

3D correlative imaging with unparalleled

accuracy of image alignment. The versatility of

LiteScope proves its applicability in a variety of

fields such as Material Science, Nanotechnology,

Semiconductors, Solar cell development, Life

Science and other areas of research as well as

industrial applications.

Material Science

  • 1D / 2D materials
  • Steel & metal alloys
  • Batteries
  • CeramicsPolymers & Composites

Semiconductors

  • Integrated circuits
  • Solar cells
  • Failure analyses
  • Dopant visualization
  • Current leakage
  • localization

Nanostructures

  • Modified surfaces FIB/GIS
  • Quantum dots
  • Nanostructured films
  • Nano-patterning
  • Nanowires

Life Science

  • Cell biology
  • Marine biology
  • Protein technology
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